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Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
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Chapitres
- 00:00Titre
- 01:03Introduction
- 02:16Constructing the Stereotaxic Clamp
- 04:08Preparing the System for the Customized Injector Array: Hamilton Pump and Stereotax
- 05:13Constructing the Customized Injector Array
- 07:25Assembling the Entire System
- 08:36Injecting the Virus
- 12:22Results of Viral Injection of the Brain
- 12:59Conclusion
Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
