Picrosirius Red Polarization Staining: A Technique To Detect Fibrosis in Cardiac Tissue Sections Using Bright-Field Microscopy

Published: April 30, 2023

Abstract

Source: Ku, H. C. et al., A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats. J. Vis. Exp. (2016). 

This video describes the picrosirius red staining of fibrotic tissues to detect excessive collagen deposition in fibrotic tissue sections. The staining technique helps assess collagen expression in connective-tissue diseases.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Tissue Fibrosis Quantification Place the paraformaldehyde-fixed heart tissue on a tissue sectioning device and cut 2 mm thick sections. Place the tissue sections in an embedding cassette. Dehydrate the tissue through a series of graded alcohol baths (50%, 75%, 95%, and 100% for 1 hr each). Infiltrate the tissu…

Divulgations

The authors have nothing to disclose.

Materials

Paraformaldehyde  Sigma Aldrich  441244
Picrosirius red solution  Abcam  ab150681
Imagequant  Molecular Dynamics

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Picrosirius Red Polarization Staining: A Technique To Detect Fibrosis in Cardiac Tissue Sections Using Bright-Field Microscopy. J. Vis. Exp. (Pending Publication), e21055, doi: (2023).

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