Picrosirius Red Polarization Staining: A Technique To Detect Fibrosis in Cardiac Tissue Sections Using Bright-Field Microscopy
Published: April 30, 2023
Abstract
Source: Ku, H. C. et al., A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats. J. Vis. Exp. (2016).
This video describes the picrosirius red staining of fibrotic tissues to detect excessive collagen deposition in fibrotic tissue sections. The staining technique helps assess collagen expression in connective-tissue diseases.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Tissue Fibrosis Quantification Place the paraformaldehyde-fixed heart tissue on a tissue sectioning device and cut 2 mm thick sections. Place the tissue sections in an embedding cassette. Dehydrate the tissue through a series of graded alcohol baths (50%, 75%, 95%, and 100% for 1 hr each). Infiltrate the tissu…
Divulgations
The authors have nothing to disclose.
Materials
| Paraformaldehyde | Sigma Aldrich | 441244 | |
| Picrosirius red solution | Abcam | ab150681 | |
| Imagequant | Molecular Dynamics |
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Picrosirius Red Polarization Staining: A Technique To Detect Fibrosis in Cardiac Tissue Sections Using Bright-Field Microscopy. J. Vis. Exp. (Pending Publication), e21055, doi: (2023).