Mouse Inguinal Lymph Node Volume Analysis: An Ultra-high-frequency Ultrasound Imaging Technique to Determine the Volume of Inguinal Lymph Node in Mouse Model

Published: April 30, 2023

Abstract

Source: Vitiello, M. et. al., Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma. J. Vis. Exp. (2021)

In this video, we demonstrate the technique to image the inguinal lymph node in a mouse model using ultra-high-frequency ultrasound imaging. This non-invasive imaging technique allows the monitoring of the change in lymph node volume in the animal over time.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Imaging procedure Place the mouse in an induction chamber for gas anesthesia and supply 3% isoflurane in pure oxygen until the animal is fully anesthetized. Verify the depth of anesthesia by lack of response to paw pinch. Transfer the animal to a heated board – a constitutive part of the UHFUS imaging station …

Divulgations

The authors have nothing to disclose.

Materials

B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ (Braf/Pten) mice The Jackson Laboratory 13590
Blu gel Sooft Ialia Ophthalmic solution gel
IsoFlo (isoflorane) Zoetis Liquid for gaseous anaesthesia
Sigma gel Parker Electrode gel
Transonic gel clear Telic SAU Ultrasound gel
Veet Reckitt Benckiser IT Depilatory cream
Compact Dual Anesthesia System Fujifilm, Visualsonics Inc. Isoflurane-based anesthesia system equipped with nose cone and induction chamber
MX550S Fujifilm, Visualsonics Inc. UHFUS linear probe
Vevo 3100 Fujifilm, Visualsonics Inc. UHFUS system
Vevo Imaging Station Fujifilm, Visualsonics Inc. UHFUS imaging station and Advancing Physiological Monitoring Unit endowed with heated board
Vevo Lab  Fujifilm, Visualsonics Inc. Software platform for ultrasound image post-processing

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Citer Cet Article
Mouse Inguinal Lymph Node Volume Analysis: An Ultra-high-frequency Ultrasound Imaging Technique to Determine the Volume of Inguinal Lymph Node in Mouse Model. J. Vis. Exp. (Pending Publication), e20799, doi: (2023).

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