Mouse Inguinal Lymph Node Volume Analysis: An Ultra-high-frequency Ultrasound Imaging Technique to Determine the Volume of Inguinal Lymph Node in Mouse Model
Published: April 30, 2023
Abstract
Source: Vitiello, M. et. al., Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma. J. Vis. Exp. (2021)
In this video, we demonstrate the technique to image the inguinal lymph node in a mouse model using ultra-high-frequency ultrasound imaging. This non-invasive imaging technique allows the monitoring of the change in lymph node volume in the animal over time.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Imaging procedure Place the mouse in an induction chamber for gas anesthesia and supply 3% isoflurane in pure oxygen until the animal is fully anesthetized. Verify the depth of anesthesia by lack of response to paw pinch. Transfer the animal to a heated board – a constitutive part of the UHFUS imaging station …
Divulgations
The authors have nothing to disclose.
Materials
| B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ (Braf/Pten) mice | The Jackson Laboratory | 13590 | |
| Blu gel | Sooft Ialia | Ophthalmic solution gel | |
| IsoFlo (isoflorane) | Zoetis | Liquid for gaseous anaesthesia | |
| Sigma gel | Parker | Electrode gel | |
| Transonic gel clear | Telic SAU | Ultrasound gel | |
| Veet | Reckitt Benckiser IT | Depilatory cream | |
| Compact Dual Anesthesia System | Fujifilm, Visualsonics Inc. | Isoflurane-based anesthesia system equipped with nose cone and induction chamber | |
| MX550S | Fujifilm, Visualsonics Inc. | UHFUS linear probe | |
| Vevo 3100 | Fujifilm, Visualsonics Inc. | UHFUS system | |
| Vevo Imaging Station | Fujifilm, Visualsonics Inc. | UHFUS imaging station and Advancing Physiological Monitoring Unit endowed with heated board | |
| Vevo Lab | Fujifilm, Visualsonics Inc. | Software platform for ultrasound image post-processing |
Tags
Citer Cet Article
Mouse Inguinal Lymph Node Volume Analysis: An Ultra-high-frequency Ultrasound Imaging Technique to Determine the Volume of Inguinal Lymph Node in Mouse Model. J. Vis. Exp. (Pending Publication), e20799, doi: (2023).