JOVE JOURNAL

Immunologie et infection

Showing 1 - 12 in 1,529 videos.

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Journal (Immunology and Infection)

In Vitro Induction of Pathogenic Th17 Cells into Naive CD4⁺ T-Cells of Mice

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Isolation and Preparation of Mice Splenic Single-Cell Suspension for Flow Cytometry Analysis

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Purification and Analysis of Mice Naive CD4⁺ T-Cells Using Magnetic Bead-Based Negative Selection

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Engineering of Human Blood-Induced Microglia-like Cells for Reverse-Translational Brain Research

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Isolation of Mononuclear Cells from Whole Blood to Obtain Induced Microglia-Like Cells for Brain Research

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Obtaining Induced Microglia-Like Cells from Whole-Blood Derived Monocytes for Probing Microglial Dynamics in Human Diseases

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Generating Single-Cell Suspension from Lung Tissue for Identifying Rare Antigen-Specific T-Cell Populations

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Enriching the Lung Sample for T Cells via Magnetic Bead Enrichment

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Staining the Antigen-Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Electronic von Frey associated with the Mouse Grimace Scale to Assess Allodynia and Pain in Trypanosoma evansi-Infected Mice

Daniel Sérgio Cipriani¹, Gabriela Kaiser Borges², Luiz Claudio Miletti², Amanda Leite Bastos-Pereira^2,3

¹Residency Program in Veterinary Medicine,Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology,Santa Catarina State University, ³Federal University of Santa Catarina

Journal (Immunology and Infection)

Preparation and Inoculation of Trypanosoma evansi in Mouse to Evaluate Allodynia and Pain

Daniel Sérgio Cipriani¹, Gabriela Kaiser Borges², Luiz Claudio Miletti², Amanda Leite Bastos-Pereira^2,3

¹Residency Program in Veterinary Medicine,Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology,Santa Catarina State University, ³Federal University of Santa Catarina

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