JOVE JOURNAL

Imunologia e Infecção

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Journal (Immunology and Infection)

Isolation and Preparation of Mice Splenic Single-Cell Suspension for Flow Cytometry Analysis

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Purification and Analysis of Mice Naive CD4⁺ T-Cells Using Magnetic Bead-Based Negative Selection

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

In Vitro Induction of Pathogenic Th17 Cells into Naive CD4⁺ T-Cells of Mice

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Isolation of Mononuclear Cells from Whole Blood to Obtain Induced Microglia-Like Cells for Brain Research

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Obtaining Induced Microglia-Like Cells from Whole-Blood Derived Monocytes for Probing Microglial Dynamics in Human Diseases

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Engineering of Human Blood-Induced Microglia-like Cells for Reverse-Translational Brain Research

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Generating Single-Cell Suspension from Lung Tissue for Identifying Rare Antigen-Specific T-Cell Populations

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Enriching the Lung Sample for T Cells via Magnetic Bead Enrichment

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Staining the Antigen-Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Grimace Scale Assessment of Mice Infected with Trypanosoma evansi

Daniel Sérgio Cipriani¹, Gabriela Kaiser Borges², Luiz Claudio Miletti², Amanda Leite Bastos-Pereira^2,3

¹Residency Program in Veterinary Medicine,Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology,Santa Catarina State University, ³Federal University of Santa Catarina

Journal (Immunology and Infection)

Electronic von Frey associated with the Mouse Grimace Scale to Assess Allodynia and Pain in Trypanosoma evansi-Infected Mice

Daniel Sérgio Cipriani¹, Gabriela Kaiser Borges², Luiz Claudio Miletti², Amanda Leite Bastos-Pereira^2,3

¹Residency Program in Veterinary Medicine,Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology,Santa Catarina State University, ³Federal University of Santa Catarina

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