JOVE JOURNAL

Immunologie et infection

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Journal (Immunology and Infection)

Preparation of Functionalized Magnetic Nanoparticles for Time-Resolved Fluorescent Imaging

Aline Linder*¹, Kevin Portmann*¹, Luca Johannes Schlotheuber¹, Alessandro Streuli¹, Wiona Sophie Glänzer¹, Klaus Eyer^1,2, Ines Lüchtefeld^1,3

¹Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences,ETH Zürich, ²Department of Biomedicine,Aarhus University, ³Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology,ETH Zürich

Journal (Immunology and Infection)

Encapsulation of the Cells and Nanoparticles into Droplets for Fluorescent Imaging

Aline Linder*¹, Kevin Portmann*¹, Luca Johannes Schlotheuber¹, Alessandro Streuli¹, Wiona Sophie Glänzer¹, Klaus Eyer^1,2, Ines Lüchtefeld^1,3

¹Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences,ETH Zürich, ²Department of Biomedicine,Aarhus University, ³Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology,ETH Zürich

Journal (Immunology and Infection)

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Aline Linder*¹, Kevin Portmann*¹, Luca Johannes Schlotheuber¹, Alessandro Streuli¹, Wiona Sophie Glänzer¹, Klaus Eyer^1,2, Ines Lüchtefeld^1,3

¹Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences,ETH Zürich, ²Department of Biomedicine,Aarhus University, ³Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology,ETH Zürich

Journal (Immunology and Infection)

Organ-Wide Detection of Pathogenic Bacteria in Infected Murine Tissue Using Tomography

Jiagui Li¹, Alaa Alhayek¹, Heng Wang¹, Dirk Bumann¹

¹Biozentrum,University of Basel

Journal (Immunology and Infection)

High-Resolution Three-Dimensional Whole-Organ Tomography of Microbial Infections

Jiagui Li¹, Alaa Alhayek¹, Heng Wang¹, Dirk Bumann¹

¹Biozentrum,University of Basel

Journal (Immunology and Infection)

Scoring Central Nervous System Inflammation, Demyelination, and Axon Injury in Experimental Autoimmune Encephalomyelitis

Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez^1,2, Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider^2,4, Shannon E Dunn^1,2,5

¹Department of Immunology,University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute,The Hospital for Sick Children, ⁴BARLO MS Centre,St. Michael’s Hospital, ⁵Women’s College Research Institute,Women’s College Hospital

Journal (Immunology and Infection)

Harvesting and Fixing Mouse Brain and Spinal Cord Tissues for Histological Scoring of Experimental Autoimmune Encephalomyelitis

Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez^1,2, Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider^2,4, Shannon E Dunn^1,2,5

¹Department of Immunology,University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute,The Hospital for Sick Children, ⁴BARLO MS Centre,St. Michael’s Hospital, ⁵Women’s College Research Institute,Women’s College Hospital

Journal (Immunology and Infection)

Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues

Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez^1,2, Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider^2,4, Shannon E Dunn^1,2,5

¹Department of Immunology,University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute,The Hospital for Sick Children, ⁴BARLO MS Centre,St. Michael’s Hospital, ⁵Women’s College Research Institute,Women’s College Hospital

Journal (Immunology and Infection)

Mouse Splenic Single-Cell Suspension Generation and FACS Staining for Flow Cytometry-Based Sorting

Katharina M. Glaser*¹, Mirijam Egg*¹, Sebastian Hobitz¹, Michael Mitterer¹, Dominik Schain-Zota¹, Katharina Schönberger¹, Konrad Schuldes¹, Nina Cabezas-Wallscheid¹, Tim Lämmermann¹, Angelika Rambold¹, Joerg M. Buescher¹

¹Max Planck Institute of Immunobiology and Epigenetics

Journal (Immunology and Infection)

FACS Sorting of Mouse Splenic Cells and Metabolite Analysis Using LC-MS

Katharina M. Glaser*¹, Mirijam Egg*¹, Sebastian Hobitz¹, Michael Mitterer¹, Dominik Schain-Zota¹, Katharina Schönberger¹, Konrad Schuldes¹, Nina Cabezas-Wallscheid¹, Tim Lämmermann¹, Angelika Rambold¹, Joerg M. Buescher¹

¹Max Planck Institute of Immunobiology and Epigenetics

Journal (Immunology and Infection)

Targeted Metabolomics on Rare Primary Cells

Katharina M. Glaser*¹, Mirijam Egg*¹, Sebastian Hobitz¹, Michael Mitterer¹, Dominik Schain-Zota¹, Katharina Schönberger¹, Konrad Schuldes¹, Nina Cabezas-Wallscheid¹, Tim Lämmermann¹, Angelika Rambold¹, Joerg M. Buescher¹

¹Max Planck Institute of Immunobiology and Epigenetics

Journal (Immunology and Infection)

Preparation and Characterization of Cationic Nanoemulsion-Encapsulated Retinoic Acid

Guo-Cheng Li¹, Hui-Fang Li¹, Zhe Jin¹, Rang Feng¹, Yan Deng¹, Hao Cheng¹, Hai-Bo Li¹

¹National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy,Third Military Medical University

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