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암 세포주에서 유래한 낮은 내독소 함량 세포외 소포의 분리
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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암 세포주에서 유래한 낮은 내독소 함량 세포외 소포의 분리

789 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

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06:28 min
February 17, 2023

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4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Summary

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제안된 프로토콜에는 세포 배양 상층액에서 세포외 소포를 분리하는 동안 내독소 오염을 방지하는 방법과 이를 적절하게 평가하는 방법에 대한 지침이 포함되어 있습니다.

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Cite this Article

Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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