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用于追踪斑马鱼径向胶质细胞祖细胞不对称分裂期间缺口/δ内吞作用的抗体摄取测定

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JoVE Revista Biología del desarrollo

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

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用于追踪斑马鱼径向胶质细胞祖细胞不对称分裂期间缺口/δ内吞作用的抗体摄取测定

DOI:

10.3791/65030-v

•

09:06 min

•

January 20, 2023

•

Xiang Zhao², Su Guo³

¹Chan Zuckerberg Biohub, ²Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, ³Programs in Human Genetics and Biological Sciences,University of California San Francisco

Capítulos

00:04Introduction
01:01Preparation of Zebrafish Embryos
02:10Preparation of Microinjection
03:51Microinjection
06:02Mounting and Time‐Lapse Live Imaging
07:32Results: Imaging Anti‐Dld‐Atto‐647N Antibody Uptake in the Developing Zebrafish Brain
08:26Conclusion

Summary

Traducción Automática

这项工作开发了一种抗体摄取测定法，用于在胚胎斑马鱼大脑的径向胶质细胞祖细胞分裂中成像谱系内Notch/DeltaD信号传导。

Tags

Antibody Uptake Assay Notch/Delta Endocytosis Asymmetric Division Zebrafish Radial Glia Progenitors Notch Signaling Sulfate Decision Patterning Development Metazoans Anti-Dld Antibody Anti-mouse IgG Atto-647N Antibody Brain Ventricles Embryonic Development Microinjection Fluorescence Microscopy

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