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用于追踪斑马鱼径向胶质细胞祖细胞不对称分裂期间缺口/δ内吞作用的抗体摄取测定
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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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用于追踪斑马鱼径向胶质细胞祖细胞不对称分裂期间缺口/δ内吞作用的抗体摄取测定

1,784 Views

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09:06 min

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January 20, 2023

DOI:

10.3791/65030-v

DOI:
10.3791/65030-v

•

09:06 min
January 20, 2023

•

2 Views
,
1Chan Zuckerberg Biohub, 2Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 3Programs in Human Genetics and Biological Sciences,University of California San Francisco

Summary

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这项工作开发了一种抗体摄取测定法，用于在胚胎斑马鱼大脑的径向胶质细胞祖细胞分裂中成像谱系内Notch/DeltaD信号传导。

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Zhao, X., Guo, S. Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors. J. Vis. Exp. (191), e65030, doi:10.3791/65030 (2023).

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