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Subcellular Imaging of Neuronal Calcium Handling In Vivo
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JoVE Revista Neurociencias
Subcellular Imaging of Neuronal Calcium Handling In Vivo

Subcellular Imaging of Neuronal Calcium Handling In Vivo

DOI:
10.3791/64928-v

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07:14 min

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March 17, 2023

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, , ,
1Department of Biomedical Sciences,Colorado State University College of Veterinary Medicine and Biomedical Sciences, 2Cellular and Molecular Biology Graduate Program,Colorado State University College of Veterinary Medicine and Biomedical Sciences

Capítulos

  • 00:04Introduction
  • 00:51Creating Transgenic Strains
  • 01:47Optical Setup
  • 02:21Preparation of Worms for Imaging
  • 04:14Acquisition of High‐Resolution Image Streams
  • 04:52Results: Rapid Imaging of Calcium Uptake in AVA Neurite
  • 06:19Conclusion

Summary

Traducción Automática

Traducción Automática

The current methods describe a non-ratiometric approach for high-resolution, sub-compartmental calcium imaging in vivo de Caenorhabditis elegans using readily available genetically encoded calcium indicators.

Tags

Keywords: Subcellular ImagingNeuronal Calcium HandlingIn VivoC. ElegansCalcium IndicatorTransgenic StrainsTime-lapse ImagingAgar PadsFluorescent ToolsCalcium SignalingNeurodegeneration

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