The current methods describe a non-ratiometric approach for high-resolution, sub-compartmental calcium imaging in vivo de Caenorhabditis elegans using readily available genetically encoded calcium indicators.
Doser, R., Knight, K. M., Deihl, E., Hoerndli, F. Subcellular Imaging of Neuronal Calcium Handling In Vivo. J. Vis. Exp. (193), e64928, doi:10.3791/64928 (2023).