Journal
/
神经科学
/
Subcellular Imaging of Neuronal Calcium Handling In Vivo
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Subcellular Imaging of Neuronal Calcium Handling In Vivo

Subcellular Imaging of Neuronal Calcium Handling In Vivo

DOI:
10.3791/64928-v

•

07:14 min

•

March 17, 2023

•

, , ,
1Department of Biomedical Sciences,Colorado State University College of Veterinary Medicine and Biomedical Sciences, 2Cellular and Molecular Biology Graduate Program,Colorado State University College of Veterinary Medicine and Biomedical Sciences

Chapters

  • 00:04Introduction
  • 00:51Creating Transgenic Strains
  • 01:47Optical Setup
  • 02:21Preparation of Worms for Imaging
  • 04:14Acquisition of High‐Resolution Image Streams
  • 04:52Results: Rapid Imaging of Calcium Uptake in AVA Neurite
  • 06:19Conclusion

Summary

自动翻译

自动翻译

The current methods describe a non-ratiometric approach for high-resolution, sub-compartmental calcium imaging in vivo in Caenorhabditis elegans using readily available genetically encoded calcium indicators.

Tags

Keywords: Subcellular ImagingNeuronal Calcium HandlingIn VivoC. ElegansCalcium IndicatorTransgenic StrainsTime-lapse ImagingAgar PadsFluorescent ToolsCalcium SignalingNeurodegeneration

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/64928?article_type=v

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
check_url/cn/64928?article_type=v

In vivo Neuronal Calcium Imaging in C. elegans
check_url/cn/64928?article_type=v

Super-resolution Imaging of Neuronal Dense-core Vesicles
check_url/cn/64928?article_type=v

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans
check_url/cn/64928?article_type=v

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
check_url/cn/64928?article_type=v

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
check_url/cn/64928?article_type=v

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
check_url/cn/64928?article_type=v

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
check_url/cn/64928?article_type=v

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity
check_url/cn/64928?article_type=v

In vivo Calcium Imaging in Mouse Inferior Olive

Read Article