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从快速冷冻肝组织中分离细胞核用于单细胞多组学

JoVE Revista
Biología

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JoVE Revista Biología

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

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从快速冷冻肝组织中分离细胞核用于单细胞多组学

DOI:

10.3791/64792-v

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09:09 min

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December 09, 2022

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Mateusz Strzelecki⁴, Kelvin Yin, Carlos Talavera-López³, Celia P. Martinez-Jimenez⁴

¹Helmholtz Pioneer Campus (HPC),Helmholtz Munich, ²Institute of Computational Biology, Computational Health Department,Helmholtz Munich, ³Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, ⁴TUM School of Medicine,Technical University of Munich

Capítulos

00:04Introduction
00:57Nuclei Isolation
05:11Nuclei Sorting for Hepatocyte Ploidy Profiling or Well-Based Sequencing Approaches
06:36Results: Isolation of Nuclei from Flash-Frozen, Archived Liver Tissues for Single-Nucleus RNA-Seq, ATAC-Seq, and Joint Multiomics
08:39Conclusion

Summary

Traducción Automática

在这里，我们提出了一种从快速冷冻，存档的肝组织中分离细胞核的方案，用于单核RNA-seq，ATAC-seq和联合多组学（RNA-seq和ATAC-seq）。

Tags

Nuclei Isolation Single-cell Multiomics Density Gradient Centrifugation Iodixanol Cell Strainer Bio-archived Human Liver Samples Mouse Model Non-human Primate

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