Here, we present a protocol to evaluate the antibacterial efficacy of an antibiotic-eluting polymer to simulate prophylactic clinical application by using a commercially available real-time ATP-based luminescent microbial viability assay. This method enables the monitoring of the longitudinal activity of drug-eluting materials and can be widely adapted to test anti-microbial drug delivery platforms.
Sekar, A., Lekkala, S., Oral, E. A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials. J. Vis. Exp. (193), e64641, doi:10.3791/64641 (2023).