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使用双光镊和微流体进行单分子研究
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
DOI:

06:53 min

November 18, 2022

Capítulos

  • 00:05Introduction
  • 01:16Flow Cell Connections Using a PDMS Flow Cell
  • 02:13Attachment of Press Fit Tubing Connectors
  • 02:50Attachment of Permanent Assemblies
  • 03:39Flow Cell Connections and Preparation
  • 04:19Results: The Power Output of the Laser, Fluid Flow Characteristics through a Glass Flow Cell, and Effects on Bead Motion and Opposing Force by Fluid Flow
  • 05:59Conclusion

Summary

Traducción Automática

使用玻璃桶、气密注射器、管道与流通池的稳定连接以及通过在注射器和管道之间放置切换阀来消除气泡,极大地促进了通过微流体室研究的视觉单分子生物化学。该协议描述了双光学陷阱,能够可视化DNA交易和分子间相互作用。

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