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Inmunología y contagio
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Culturing and Genetically Manipulating Entomopathogenic Nematodes
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Culturing and Genetically Manipulating Entomopathogenic Nematodes

Culturing and Genetically Manipulating Entomopathogenic Nematodes

DOI:
10.3791/63885-v

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07:17 min

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March 31, 2022

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, , , ,
1Department of Biological Sciences,The George Washington University, 2Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences,The George Washington University

Capítulos

  • 00:04Introduction
  • 00:36Production of Symbiotic Nematode Infective Juveniles
  • 02:27Heterorhabditis bacteriophora Infective Juveniles and Surface Sterilizing
  • 05:55Results: Validation of Heterorhabditis bacteriophora Axenization
  • 06:24Conclusion

Summary

Traducción Automática

Traducción Automática

Entomopathogenic nematodes live in symbiosis with bacteria and together they successfully infect insects by undermining their innate immune system. To promote research on the genetic basis of nematode infection, methods for maintaining and genetically manipulating entomopathogenic nematodes are described.

Tags

Entomopathogenic NematodesHeterorhabditis BacteriophoraSteinernema CarpocapsaeGalleria MellonellaInfective JuvenilesWhite’s Water TrapsPhotorhabdus TemperataMacConkey PlateLB BrothIn Vitro CulturingGenetic Manipulation

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