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Culturing and Genetically Manipulating Entomopathogenic Nematodes
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Culturing and Genetically Manipulating Entomopathogenic Nematodes

Culturing and Genetically Manipulating Entomopathogenic Nematodes

2,959 Views

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07:17 min

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March 31, 2022

DOI:

10.3791/63885-v

DOI:
10.3791/63885-v

•

07:17 min
March 31, 2022

•

8 Views
, , , ,
1Department of Biological Sciences,The George Washington University, 2Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences,The George Washington University

Summary

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Entomopathogenic nematodes live in symbiosis with bacteria and together they successfully infect insects by undermining their innate immune system. To promote research on the genetic basis of nematode infection, methods for maintaining and genetically manipulating entomopathogenic nematodes are described.

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Cite this Article

Heryanto, C., Ratnappan, R., O'Halloran, D. M., Hawdon, J. M., Eleftherianos, I. Culturing and Genetically Manipulating Entomopathogenic Nematodes. J. Vis. Exp. (181), e63885, doi:10.3791/63885 (2022).

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