需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Culturing and Genetically Manipulating Entomopathogenic Nematodes
Chapters
- 00:04Introduction
- 00:36Production of Symbiotic Nematode Infective Juveniles
- 02:27Heterorhabditis bacteriophora Infective Juveniles and Surface Sterilizing
- 05:55Results: Validation of Heterorhabditis bacteriophora Axenization
- 06:24Conclusion
Entomopathogenic nematodes live in symbiosis with bacteria and together they successfully infect insects by undermining their innate immune system. To promote research on the genetic basis of nematode infection, methods for maintaining and genetically manipulating entomopathogenic nematodes are described.
