Journal
/
免疫与感染
/
Culturing and Genetically Manipulating Entomopathogenic Nematodes
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Culturing and Genetically Manipulating Entomopathogenic Nematodes

Culturing and Genetically Manipulating Entomopathogenic Nematodes

2,959 Views

•

07:17 min

•

March 31, 2022

DOI:

10.3791/63885-v

DOI:
10.3791/63885-v

•

07:17 min
March 31, 2022

•

8 Views
, , , ,
1Department of Biological Sciences,The George Washington University, 2Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences,The George Washington University

Summary

Automatically generated

Entomopathogenic nematodes live in symbiosis with bacteria and together they successfully infect insects by undermining their innate immune system. To promote research on the genetic basis of nematode infection, methods for maintaining and genetically manipulating entomopathogenic nematodes are described.

Explore More Videos

Entomopathogenic NematodesHeterorhabditis BacteriophoraSteinernema CarpocapsaeGalleria MellonellaInfective JuvenilesWhite’s Water TrapsPhotorhabdus TemperataMacConkey PlateLB BrothIn Vitro CulturingGenetic Manipulation

Read Article

Cite this Article

Heryanto, C., Ratnappan, R., O'Halloran, D. M., Hawdon, J. M., Eleftherianos, I. Culturing and Genetically Manipulating Entomopathogenic Nematodes. J. Vis. Exp. (181), e63885, doi:10.3791/63885 (2022).

Download .ris file

Copy

Share Video

.

Copy