Journal
/
Bioquímica
/
通过高分辨率呼吸测量法评估坐骨神经的线粒体功能
JoVE Revista
Bioquímica
Author Produced
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
Please note that all translations are automatically generated. Click here for the English version.

通过高分辨率呼吸测量法评估坐骨神经的线粒体功能

2,134 Views

•

08:19 min

•

May 05, 2022

DOI:

10.3791/63690-v

DOI:
10.3791/63690-v

•

08:19 min
May 05, 2022

•

11 Views
,
1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Summary

Automatically generated

高分辨率呼吸测量与荧光传感器相结合，可测定线粒体耗氧量和活性氧 （ROS） 生成。本方案描述了一种评估透化坐骨神经中线粒体呼吸频率和ROS产生的技术。

Explore More Videos

Mitochondrial FunctionSciatic NerveHigh-resolution RespirometryOxygen ConsumptionROS ProductionNeuropathiesDiabetesChemotherapyNeurobiologyBioquímicaPharmacologyMitochondrial Disease MechanismsMitochondrial BoostersNeuropathic PainPermeabilizationAmplex RedPeroxidase

Read Article

Cite this Article

Formiga-Jr, M. A., Camacho-Pereira, J. Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry. J. Vis. Exp. (183), e63690, doi:10.3791/63690 (2022).

Download .ris file

Copy

Share Video

.

Copy