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小鼠小肠上皮类器官与先天性淋巴细胞的共培养
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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小鼠小肠上皮类器官与先天性淋巴细胞的共培养

4,706 Views

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08:22 min

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March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

•

08:22 min
March 23, 2022

•

14 Views
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1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Summary

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该协议为建立小鼠小肠类器官，从小鼠小肠固有层中分离1型先天淋巴细胞，以及在两种细胞类型之间建立3维（3D）共培养物以研究肠上皮细胞和1型先天淋巴细胞之间的双向相互作用提供了详细的说明。

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Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

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