Journal
/
Bioquímica
/
Purification of Endogenous Drosophila Transient Receptor Potential Channels
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Purification of Endogenous Drosophila Transient Receptor Potential Channels

Purification of Endogenous Drosophila Transient Receptor Potential Channels

1,546 Views

•

08:39 min

•

December 28, 2021

DOI:

10.3791/63260-v

DOI:
10.3791/63260-v

•

08:39 min
December 28, 2021

•

9 Views
, , , , ,
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute,Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center

Summary

Automatically generated

Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.

Explore More Videos

DrosophilaTransient Receptor Potential (TRP) ChannelsProtein PurificationGST-tagged TRPSize Exclusion ChromatographyE. Coli ExpressionINAD Protein ComplexHigh-pressure Homogenization

Read Article

Cite this Article

Liu, J., Liu, Y., Chen, W., Ding, Y., Lan, X., Liu, W. Purification of Endogenous Drosophila Transient Receptor Potential Channels. J. Vis. Exp. (178), e63260, doi:10.3791/63260 (2021).

Download .ris file

Copy

Share Video

.

Copy