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Purification of Endogenous Drosophila Transient Receptor Potential Channels
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JoVE Journal Biochemie
Purification of Endogenous Drosophila Transient Receptor Potential Channels

Purification of Endogenous Drosophila Transient Receptor Potential Channels

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08:39 min

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December 28, 2021

DOI:

10.3791/63260-v

DOI:
10.3791/63260-v

•

08:39 min
December 28, 2021

•

9 Views
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1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute,Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center

Summary

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Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.

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DrosophilaTransient Receptor Potential (TRP) ChannelsProtein PurificationGST-tagged TRPSize Exclusion ChromatographyE. Coli ExpressionINAD Protein ComplexHigh-pressure Homogenization

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Liu, J., Liu, Y., Chen, W., Ding, Y., Lan, X., Liu, W. Purification of Endogenous Drosophila Transient Receptor Potential Channels. J. Vis. Exp. (178), e63260, doi:10.3791/63260 (2021).

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