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Using Optogenetics to Reverse Neuroplasticity and Inhibit Cocaine Seeking in Rats
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Capítulos
- 00:04Introduction
- 00:46Rodent Intravenous Catheterization, Virus Delivery, and Optic Fiber Implantation
- 04:11Rodent Cocaine Self-Administration and Instrumental Lever Extinction
- 05:25In Vivo Optogenetic Induction of Long-Term Depression (LTD)
- 06:40Test the Effect of Optogenetic Stimulation on Cue-Induced Cocaine Seeking
- 07:15Results: The Behavioral and Histological Analysis after Cocaine Self-Administration Experiments in Rats
- 09:02Conclusion
The methods described here outline a procedure used to optogenetically reverse cocaine-induced plasticity in a behaviorally-relevant circuit in rats. Sustained low-frequency optical stimulation of thalamo-amygdala synapses induces long-term depression (LTD). In vivo optogenetically-induced LTD in cocaine-experienced rats resulted in the subsequent attenuation of cue-motivated drug seeking.
