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Using Optogenetics to Reverse Neuroplasticity and Inhibit Cocaine Seeking in Rats
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JoVE 杂志 神经科学
Using Optogenetics to Reverse Neuroplasticity and Inhibit Cocaine Seeking in Rats

Using Optogenetics to Reverse Neuroplasticity and Inhibit Cocaine Seeking in Rats

DOI:
10.3791/63185-v

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09:43 min

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October 05, 2021

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, ,
1Department of Psychiatry,University of Pittsburgh, 2Department of Psychiatry,Rutgers University

Chapters

  • 00:04Introduction
  • 00:46Rodent Intravenous Catheterization, Virus Delivery, and Optic Fiber Implantation
  • 04:11Rodent Cocaine Self-Administration and Instrumental Lever Extinction
  • 05:25In Vivo Optogenetic Induction of Long-Term Depression (LTD)
  • 06:40Test the Effect of Optogenetic Stimulation on Cue-Induced Cocaine Seeking
  • 07:15Results: The Behavioral and Histological Analysis after Cocaine Self-Administration Experiments in Rats
  • 09:02Conclusion

Summary

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自动翻译

The methods described here outline a procedure used to optogenetically reverse cocaine-induced plasticity in a behaviorally-relevant circuit in rats. Sustained low-frequency optical stimulation of thalamo-amygdala synapses induces long-term depression (LTD). In vivo optogenetically-induced LTD in cocaine-experienced rats resulted in the subsequent attenuation of cue-motivated drug seeking.

Tags

Keywords: OptogeneticsNeuroplasticityCocaine SeekingRatsAAVMedial Geniculate NucleusIntravenous CatheterStereotaxic Surgery神经科学Addiction Research

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