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DNA二重鎖破断時の転写活性の生細胞イメージング
JoVE Revista
Biología
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JoVE Revista Biología
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
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DNA二重鎖破断時の転写活性の生細胞イメージング

2,348 Views

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09:07 min

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September 20, 2021

DOI:

10.3791/62968-v

DOI:
10.3791/62968-v

•

09:07 min
September 20, 2021

•

5 Views
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1Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Summary

Automatically generated

このプロトコルは、新しいレポーター遺伝子システムと、一分子感受性を有するDNA二本鎖破断時の転写を検出するための実験的セットアップを提示する。

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de Almeida, M. R., Gameiro, E., de Almeida, S. F., Martin, R. M. Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks. J. Vis. Exp. (175), e62968, doi:10.3791/62968 (2021).

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