Journal
/
Biología
/
Live-Cell Imaging van transcriptionele activiteit bij DNA Double-Strand Breaks
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
Please note that all translations are automatically generated. Click here for the English version.

Live-Cell Imaging van transcriptionele activiteit bij DNA Double-Strand Breaks

2,348 Views

•

09:07 min

•

September 20, 2021

DOI:

10.3791/62968-v

DOI:
10.3791/62968-v

•

09:07 min
September 20, 2021

•

5 Views
, , ,
1Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Summary

Automatically generated

Dit protocol presenteert een nieuw reporter-gensysteem en de experimentele opstelling om transcriptie te detecteren bij DNA-dubbelstrengsbreuken met gevoeligheid van één molecuul.

Explore More Videos

Live-cell ImagingTranscriptional ActivityDNA Double-strand BreaksTranscription DetectionSingle-cell AnalysisDNA Damage ResponseTranscription-replication CrosstalkDoxycycline InductionCalibration MeasurementsMicrocentrifuge Tube PreparationTransfectionGlass Bottom DishMicroscopy Observation

Read Article

Cite this Article

de Almeida, M. R., Gameiro, E., de Almeida, S. F., Martin, R. M. Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks. J. Vis. Exp. (175), e62968, doi:10.3791/62968 (2021).

Download .ris file

Copy

Share Video

.

Copy