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从全血和巴菲皮脂中分离出人类嗜中性粒细胞
JoVE Revista
Biología
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JoVE Revista Biología
Isolation of Human Neutrophils from Whole Blood and Buffy Coats
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从全血和巴菲皮脂中分离出人类嗜中性粒细胞

DOI:
10.3791/62837-v

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08:12 min

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September 17, 2021

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, , ,
1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

Capítulos

  • 00:04Introduction
  • 00:45Gradient Layering
  • 02:40Erythrocyte Sedimentation
  • 03:45Residual RBC Lysis
  • 05:06Quality Determination of Isolated Neutrophil
  • 05:44Results: Neutrophil Yield and Quality Estimation
  • 07:34Conclusion

Summary

Traducción Automática

Traducción Automática

该协议详细介绍了从全血，黄褐色涂层或白细胞分离膜中性粒细胞的方法，从而实现良好的产量，高纯度和最小的细胞活化。我们利用梯度纯化，红细胞（RBC）沉降和红细胞裂解来获得高质量/纯度的中性粒细胞制剂。

Tags

NeutrophilsDensity GradientSedimentationLysisBuffy CoatPBMCErythrocyteCell BiologyCell IsolationCell Preparation

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