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Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

DOI:
10.3791/62725-v

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14:22 min

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November 20, 2021

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, , ,
1College of Pharmacy, Rady Faculty of Health Sciences,University of Manitoba

Summary

Traducción Automática

Traducción Automática

This protocol presents steps to acquire and analyze fluorescent calcium images from brain ensheathing pericytes and blood flow data from nearby blood vessels in anesthetized mice. These techniques are useful for studies of mural cell physiology and can be adapted to investigate calcium transients in any cell type.

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Brain PericyteCalcium ImagingHemodynamic ImagingTransgenic MiceActa2-RCaMP 1.07Cranial WindowTail Vein InjectionFluorescein Dextran DyeKetamine Xylazine AnesthesiaTwo-photon Imaging

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