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Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

DOI:
10.3791/62725-v

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14:22 min

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November 20, 2021

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, , ,
1College of Pharmacy, Rady Faculty of Health Sciences,University of Manitoba

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol presents steps to acquire and analyze fluorescent calcium images from brain ensheathing pericytes and blood flow data from nearby blood vessels in anesthetized mice. These techniques are useful for studies of mural cell physiology and can be adapted to investigate calcium transients in any cell type.

Tags

Brain PericyteCalcium ImagingHemodynamic ImagingTransgenic MiceActa2-RCaMP 1.07Cranial WindowTail Vein InjectionFluorescein Dextran DyeKetamine Xylazine AnesthesiaTwo-photon Imaging

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