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Preparação de Vírus da Influenza Aviária H5 Pseudotipados com Método de Transfecção de Fosfato de Cálcio e Mensuração da Atividade Neutralizante de Anticorpos
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Preparação de Vírus da Influenza Aviária H5 Pseudotipados com Método de Transfecção de Fosfato de Cálcio e Mensuração da Atividade Neutralizante de Anticorpos
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology,Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital,Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,University of Chinese Academy of Sciences
Capítulos
- 00:04Introduction
- 00:40Pseudovirus Packaging with Calcium‐Phosphate Transfection
- 01:33Detection of the HA, NA, and HIV‐1 P24 Protein Expression of Influenza Pseudovirus
- 02:24Pseudovirus Titration
- 03:22Luciferase Assay
- 04:06Pseudovirus Neutralization (PN) Assay
- 05:16Results: HPAI H5 Pseudovirus Preparations and Pseudovirus Neutralization Assays
- 06:50Conclusion
