Journal
/
Bioquímica
/
Ultracentrifugation à gradient de densité pour l’étude du recyclage endocytaire dans les cellules de mammifères
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
Please note that all translations are automatically generated. Click here for the English version.

Ultracentrifugation à gradient de densité pour l’étude du recyclage endocytaire dans les cellules de mammifères

3,538 Views

•

05:13 min

•

June 30, 2021

DOI:

10.3791/62621-v

DOI:
10.3791/62621-v

•

05:13 min
June 30, 2021

•

13 Views
, ,
1School of Life Sciences,The Chinese University of Hong Kong

Tags

Density Gradient UltracentrifugationEndocytic RecyclingMammalian CellsProtein TraffickingRecycling EndosomesCell FractionationSucrose GradientPost-nuclear SupernatantHomogenization BufferDounce HomogenizerProtease InhibitorPhosphatase Inhibitor

Read Article

Cite this Article

Chan, W. W. R., Zhai, Y. Q., Lau, K. Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells. J. Vis. Exp. (172), e62621, doi:10.3791/62621 (2021).

Download .ris file

Copy

Share Video

.

Copy