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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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生化学
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JoVE Journal 生化学
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

3,574 Views

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13:36 min

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December 04, 2021

DOI:

10.3791/62522-v

DOI:
10.3791/62522-v

•

13:36 min
December 04, 2021

•

8 Views
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1Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 2Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences

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Here we present a protocol for the optimized FlashPack capillary column packing procedure. Application of an optimized protocol to a common 100-bar pressure bomb setup allows 10-times faster packing and manufacturing of long ultra-high performance capillary columns.

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Kovalchuk, S. I., Ziganshin, R., Shelukhina, I. Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach. J. Vis. Exp. (178), e62522, doi:10.3791/62522 (2021).

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