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Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
JoVE Revista
Investigación sobre el cáncer
This content is Free Access.
JoVE Revista Investigación sobre el cáncer
Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
DOI:

09:27 min

April 01, 2021

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Capítulos

  • 00:05Introduction
  • 01:08Preparation of 10% Cholesterol-Enriched Diet for Acute Cholesterol Surplus
  • 02:29NASH Induction with Short-Term Larvae Feeding with Cholesterol-Enriched Diet Static Conditions
  • 04:22Collection of 13 Days Post-Fertilization Larvae from Feeding Boxes
  • 05:06Non-Invasive Confocal Imaging Using the Zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI)
  • 05:54Immune Cell Recruitment Analysis
  • 06:49Results: Monitoring Liver Disease Progression in the Hepatocellular Carcinoma (HCC) Zebrafish Model
  • 08:30Conclusion

Summary

Traducción Automática

Here, we present how to generate a non-alcoholic fatty liver disease (NAFLD)-associated Hepatocellular Carcinoma (HCC) zebrafish model to study the impact of cholesterol surplus on liver microenvironment and immune cell landscape.

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