A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.
van Oostrom, M. J., Meijer, W. H. M., Sonnen, K. F. A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis. J. Vis. Exp. (169), e62318, doi:10.3791/62318 (2021).