Journal
/
Neurociencias
/
使用清晰组织清除方法对全山视网膜进行免疫染色
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
Please note that all translations are automatically generated. Click here for the English version.

使用清晰组织清除方法对全山视网膜进行免疫染色

6,815 Views

•

09:01 min

•

March 06, 2021

DOI:

10.3791/62178-v

DOI:
10.3791/62178-v

•

09:01 min
March 06, 2021

•

11 Views
,
1Eye Research Institute,Oakland University

Summary

Automatically generated

在这里，我们提出了一个协议，以调整整个安装视网膜脑组织的CLARITY方法，以提高标准免疫化学染色和视网膜神经元及其细胞下结构的高分辨率成像的质量。

Explore More Videos

ImmunostainingWhole-mount RetinaRetinal NeuronsMorphologyOptical Transparency3D ImagingCircuit WiringSubcellular StructuresEnucleationCorneaLensScleraRetina IsolationNitrocellulose FilterA4P0 SolutionSDSTriton X-100Primary AntibodyImmunostaining

Read Article

Cite this Article

Alessio, E. J., Zhang, D. Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method. J. Vis. Exp. (169), e62178, doi:10.3791/62178 (2021).

Download .ris file

Copy

Share Video

.

Copy