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Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology
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JoVE Revista Bioingeniería
Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

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10:41 min

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January 23, 2021

DOI:

10.3791/61962-v

DOI:
10.3791/61962-v

•

10:41 min
January 23, 2021

•

11 Views
,
1University of Newcastle, 2University of Sydney, 3Kolling Institute of Medical Research,Royal North Shore Hospital, 4University of Technology, Sydney

Summary

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This protocol aims to fabricate 3D cardiac spheroids (CSs) by co-culturing cells in hanging drops. Collagen-embedded CSs are treated with doxorubicin (DOX, a cardiotoxic agent) at physiological concentrations to model heart failure. In vitro testing using DOX-treated CSs may be used to identify novel therapies for heart failure patients.

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Cardiac SpheroidsIn VitroHuman Heart PathophysiologyCardiac MyocytesCardiac FibroblastsCardiac Endothelial CellsDrug Toxicity AssaysDoxorubicinCardiomyocytesCell CultureHanging DropSpheroid Formation

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Cite this Article

Sharma, P., Gentile, C. Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology. J. Vis. Exp. (167), e61962, doi:10.3791/61962 (2021).

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