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Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology
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Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

DOI:
10.3791/61962-v

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10:41 min

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January 23, 2021

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,
1University of Newcastle, 2University of Sydney, 3Kolling Institute of Medical Research,Royal North Shore Hospital, 4University of Technology, Sydney

Chapitres

  • 00:00Introduction
  • 00:54Cardiac Spheroid Formation
  • 05:46Drug Testing
  • 06:42Representative Results
  • 07:36Endothelial Cell Network Staining
  • 09:52Representative Results
  • 10:04Conclusion

Summary

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This protocol aims to fabricate 3D cardiac spheroids (CSs) by co-culturing cells in hanging drops. Collagen-embedded CSs are treated with doxorubicin (DOX, a cardiotoxic agent) at physiological concentrations to model heart failure. In vitro testing using DOX-treated CSs may be used to identify novel therapies for heart failure patients.

Tags

Cardiac SpheroidsIn VitroHuman Heart PathophysiologyCardiac MyocytesCardiac FibroblastsCardiac Endothelial CellsDrug Toxicity AssaysDoxorubicinCardiomyocytesCell CultureHanging DropSpheroid Formation

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