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Spot variation fluorescens korrelationsspektroskopi til analyse af molekylær diffusion ved plasmamembranen af levende celler

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot variation fluorescens korrelationsspektroskopi til analyse af molekylær diffusion ved plasmamembranen af levende celler

2,374 Views

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05:56 min

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November 12, 2020

DOI:

10.3791/61823-v

DOI:

10.3791/61823-v

•

05:56 min

November 12, 2020

•

7 Views

Sebastien Mailfert*¹, Karolina Wojtowicz*², Sophie Brustlein*¹, Ewa Blaszczak, Nicolas Bertaux, Marcin Łukaszewicz, Didier Marguet, Tomasz Trombik

¹CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, ²Faculty of Biotechnology,University of Wroclaw, ³Faculty of Biological Sciences,University of Wroclaw, ⁴CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Tags

Spot Variation Fluorescence Correlation Spectroscopy Molecular Diffusion Plasma Membrane Living Cells Confocal Microscopy Laser Power Rhodamine 6G Auto Correlation Function Diffusion Time SvFCS Analysis XY Scan Z Scan

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