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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
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生化学
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JoVE Journal 生化学
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

DOI:
10.3791/61823-v

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05:56 min

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November 12, 2020

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, , , , , , ,
1CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, 2Faculty of Biotechnology,University of Wroclaw, 3Faculty of Biological Sciences,University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

章

  • 00:05Introduction
  • 00:36Daily Checkpoint
  • 01:31Spot Size Calibration
  • 02:36svFCS Data Acquisition
  • 03:56Results: Representative svFCS Diffusion Law and Total Cholesterol Comparison
  • 05:07Conclusion

概要

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自動翻訳

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

タグ

Spot Variation Fluorescence Correlation SpectroscopyMolecular DiffusionPlasma MembraneLiving CellsConfocal MicroscopyLaser PowerRhodamine 6GAuto Correlation FunctionDiffusion TimeSvFCS AnalysisXY ScanZ Scan

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