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点变异荧光相关光谱用于分析活细胞质膜上的分子扩散
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
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点变异荧光相关光谱用于分析活细胞质膜上的分子扩散

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05:56 min

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November 12, 2020

DOI:

10.3791/61823-v

DOI:
10.3791/61823-v

•

05:56 min
November 12, 2020

•

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1CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, 2Faculty of Biotechnology,University of Wroclaw, 3Faculty of Biological Sciences,University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Tags

Spot Variation Fluorescence Correlation SpectroscopyMolecular DiffusionPlasma MembraneLiving CellsConfocal MicroscopyLaser PowerRhodamine 6GAuto Correlation FunctionDiffusion TimeSvFCS AnalysisXY ScanZ Scan

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Mailfert, S., Wojtowicz, K., Brustlein, S., Blaszczak, E., Bertaux, N., Łukaszewicz, M., Marguet, D., Trombik, T. Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells. J. Vis. Exp. (165), e61823, doi:10.3791/61823 (2020).

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