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小鼠河马兴奋和类特异性抑制神经元的不同组织型切片培养的单细胞电位

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Neurociencias

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JoVE Revista Neurociencias

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

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小鼠河马兴奋和类特异性抑制神经元的不同组织型切片培养的单细胞电位

3,381 Views

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06:21 min

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October 06, 2020

DOI:

10.3791/61662-v

DOI:

10.3791/61662-v

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06:21 min

October 06, 2020

•

3 Views

David G. Keener*^1,2, Amy Cheung*^1,3, Kensuke Futai

¹Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, ²Interdisciplinary Graduate Program,University of Massachusetts Medical School, ³UMMS MD/PhD Program,University of Massachusetts Medical School

Tags

Single-cell Electroporation Organotypic Slice Culture Mouse Hippocampus Excitatory Neurons Inhibitory Neurons Gene Transfection Neurobiology Electroporation Protocol High Transfection Efficiency Cell-autonomous Mechanisms Transsynaptic Protein Interactions Slice Culture Preparation Electroporator Setup Pipette Preparation Slice Culture Isolation Target Cell Electroporation

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