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Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
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Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

4,486 Views

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06:49 min

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May 08, 2020

DOI:

10.3791/61258-v

DOI:
10.3791/61258-v

•

06:49 min
May 08, 2020

•

1 Views
,
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Tags

Polynucleotide KinasePhosphorylationDNARNASubstrateNonradioactive AssayAcrylamide GelDenaturing GelUreaTBE BufferRNA Enzyme Kinase ReactionsATP SubstrateQuenchingDownstream Analysis

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Cite this Article

Pillon, M. C., Stanley, R. E. Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates. J. Vis. Exp. (159), e61258, doi:10.3791/61258 (2020).

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