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Tek Hücreli Genomik Uygulamalar için Yağ Dokusu Çekirdeklerinin İzolasyon
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Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
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Tek Hücreli Genomik Uygulamalar için Yağ Dokusu Çekirdeklerinin İzolasyon

9,582 Views

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07:03 min

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June 12, 2020

DOI:

10.3791/61230-v

DOI:
10.3791/61230-v

•

07:03 min
June 12, 2020

•

11 Views
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1Departments of Medicine,Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology,Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism

Summary

Automatically generated

Bu yayın, çekirdeklerin olgun adipositlerden izole edilmesi, floresan-aktive sıralama ile arınma ve tek hücre düzeyinde transkripsiyon için bir protokol açıklar.

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Cite this Article

Benitez, G. J., Shinoda, K. Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications. J. Vis. Exp. (160), e61230, doi:10.3791/61230 (2020).

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