Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:04Introduction
- 00:42Cigarette Smoking Extract (CSE) Preparation
- 01:39Pseudomonas Culture
- 02:16Cell Culture and CSE Treatment
- 03:33Bacterial Infection and Gentamycin Treatment
- 04:24Determination of Bacterial Concentration Using the Drop Plate Method
- 04:55RT-qPCR Detection of Bacterial 16S rRNA
- 07:00Results: CSE-Treatment Increases Bacterial Load
- 08:23Conclusion
