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JoVE Journal Genetics
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
 

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Article DOI: 10.3791/61060-v • 07:30 min • June 8th, 2020
June 8th, 2020 •
*1, *1,2, 1, 1, 1,2, 1
1NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research
* These authors contributed equally

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This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.

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Optimization Sequencing Analysis Degraded FFPE-RNA Samples Gene Expression Data Suboptimal Quality Samples RNA Quality Assessment Downstream Processing Sample Sequencing Data Characterization Functional Elements Of The Genome Development And Disease FFPE-RNA Degradation Sample QC Library Preparation Data Analysis RNA QC System Bioanalysis Software RNA Fragments Nucleotides
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