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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
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遗传学
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JoVE 杂志 遗传学
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

11,611 Views

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07:30 min

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June 08, 2020

DOI:

10.3791/61060-v

DOI:
10.3791/61060-v

•

07:30 min
June 08, 2020

•

3 Views
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1NCI CCR Sequencing Facility,Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences,Frederick National Laboratory for Cancer Research

Summary

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This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.

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Levin, Y., Talsania, K., Tran, B., Shetty, J., Zhao, Y., Mehta, M. Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples. J. Vis. Exp. (160), e61060, doi:10.3791/61060 (2020).

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