Journal
/
Bioquímica
/
Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins
JoVE Revista
Bioquímica
This content is Free Access.
JoVE Revista Bioquímica
Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

5,272 Views

•

09:03 min

•

March 11, 2020

DOI:

10.3791/60911-v

DOI:
10.3791/60911-v

•

09:03 min
March 11, 2020

•

4 Views
, , ,
1Department of Pharmaceutical Sciences,University of Maryland Baltimore, 2AIT Bioscience

Summary

Automatically generated

Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP).

Explore More Videos

In-cell FPOPProtein-protein InteractionsProtein Structural ChangesNative EnvironmentsMembrane ProteinsDiseasesCancerGenetic DisordersFlow SystemSyringe PumpLaser IrradiationExcimer LaserCell CultureCell Preparation

Read Article

Cite this Article

Chea, E. E., Rinas, A., Espino, J. A., Jones, L. M. Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins. J. Vis. Exp. (157), e60911, doi:10.3791/60911 (2020).

Download .ris file

Copy

Share Video

.

Copy