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生物化学
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Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

5,272 Views

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09:03 min

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March 11, 2020

DOI:

10.3791/60911-v

DOI:
10.3791/60911-v

•

09:03 min
March 11, 2020

•

4 Views
, , ,
1Department of Pharmaceutical Sciences,University of Maryland Baltimore, 2AIT Bioscience

Summary

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Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP).

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Chea, E. E., Rinas, A., Espino, J. A., Jones, L. M. Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins. J. Vis. Exp. (157), e60911, doi:10.3791/60911 (2020).

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