Journal
/
Neurociencias
/
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

DOI:
10.3791/60886-v

•

06:40 min

•

April 23, 2020

•

, , ,
1Department of Psychology,Elizabethtown College, 2Department of Psychiatry,Yale University, 3Department of Cellular and Molecular Physiology,Yale University

Capítulos

  • 00:04Introduction
  • 00:56Electrode Implantation
  • 03:12Carbon Fiber and Stimulating Electrode/Guide Cannula Location Optimization
  • 04:26Combination Infusion and Stimulation Fast-Scan Cyclic Voltammetry (FSCV) Recording
  • 05:18Results: Representative Analyses of Stimulation-Evoked Phasic Dopamine Release
  • 06:03Conclusion

Summary

Traducción Automática

Traducción Automática

The goal of this protocol is to directly manipulate ventral tegmental area receptors to study their contribution to subsecond dopamine release.

Tags

Ventral Tegmental AreaPhasic Dopamine ReleaseNucleus AccumbensReceptor FunctionCarbon Fiber MicroelectrodeBipolar StimulatorReference ElectrodeSterilizedLambdaBregmaScrewDuraBipolar Stimulating Electrode

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Voltage Biasing, Cyclic Voltammetry, & Electrical Impedance Spectroscopy for Neural Interfaces

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

Effects of Transcranial Alternating Current Stimulation on the Primary Motor Cortex by Online Combined Approach with Transcranial Magnetic Stimulation

Read Article